Additional file 13 of Altered neuronal physiology, development, and function associated with a common chromosome 15 duplication involving CHRNA7

Additional file 13. Comparison of electrophysiological properties of neurons derived in vitro from the UC-M, AP, and UM iPSC models. (.xlsx). Data is shown for (A) all cells (cExNs and cINs), (B) cExNs, and (C) cINs. (A) Data columns for cells of each genotype show the median, first, and third quartiles, and count (# of cells) for each parameter for all cells recorded, by combining data from cExNs and cINs for each genotype. Columns to the right show neuronal cell type-specific data, by combining cExN data or cIN data for all three genotypes. Light red shading denotes Significant Difference by 2-WAY ANOVA on ranks with post hoc comparison by Student-Newman-Keuls method (p < 0.05) with genotype (UC-M : AP : UM) and neuronal cell type (cExN : cIN) as the two factors. (B-C) Values for (B) cExNs or (C) cINs of each genotype are shown. Tan shading denotes Significant Difference by 1-WAY ANOVA on ranks with post hoc comparison by Student-Newman-Keuls method (p < 0.05), with darker tan indicating that one of the genotypes was different from the other two and lighter tan indicating that two of the three genotypes were different from each other. Capacitance and input resistance were determined from 10 mV voltage steps from -80 mV. First spike threshold, amplitude and half-width were determined for the first action potential recorded at threshold. Maximum first frequency and maximum average firing frequency were determined for 800 msec depolarizing steps that elicited the maximum number of spikes under current clamp. Peak inward sodium current, steady-state outward potassium currents and currents evoked by ACh and choline were recorded under whole-cell voltage clamp. Values are provided.