Transcriptional profiling of human cardiomyocytes and endothelial cells derived from NOTCH1 knockout induced pluripotent stem cells (iPSCs)

Pathogenic NOTCH1 mutations are linked to congenital heart defects. To pinpoint how NOTCH1 deficiency affects cardiac development, we generated homozygous NOTCH1 knockout (N1KO) human induced pluripotent stem cells (iPSCs). We then performed high-throughput RNA-seq to profile differential gene expression in cardiomyocytes (iPSC-CMs) and endothelial cells (iPSC-ECs) derived from wild type (WT) and N1KO iPSCs. Transcriptomic profiling of cardiomyocytes (iPSC-CMs) and endothelial cells (iPSC-ECs) derived from WT and N1KO iPSCs.