Plagl1/Zac1 regulates extracellular matrix genes and the imprinted gene network. Mus musculus

In order to determine the targets of the imprinted transcription factor Zac1, we compared wild type and Zac1-mutant MEFs and measured the differentially expressed genes by RNA-seq. Genes deregulated in the absence of Zac1 include genes belonging to the Imprinted Gene Network and genes coding for the extra-cellular matrix. Overall design: Two biological replicates of MEFs wildtype and Zac1-mutant were cultivated at different time point corresponding to proliferative and quiescent states. Genes differentially expressed between WT and Zac1-mutant MEFs were determined using RNA-seq.