Effect of Smyd1 conditional knockout on gene expression in skeletal muscle

Transcriptome analysis by RNA-seq of tibialis anterior muscle from control and Smyd1 myocyte-specific conditional knockout mice at 6 weeks of age. Smyd1 is a methyltransferase specifically expressed in striated muscle and CD8+ T cells. Smyd1 deficiency resulted in centronuclear myopathy primarily affecting fast-twitch muscle fibers. These results provide insight into how loss of Smyd1 altered transcriptional programs resulting in centronuclear myopathy. 6 animals per group (control and Smyd1 conditional knockout)本数据集针对6周龄对照组与Smyd1肌细胞特异性条件性敲除小鼠的胫前肌开展RNA测序（RNA-seq）转录组分析。Smyd1是一种特异性在横纹肌（striated muscle）与CD8+ T细胞中表达的甲基转移酶（methyltransferase）。Smyd1缺失会引发主要累及快肌纤维（fast-twitch muscle fibers）的中央核肌病（centronuclear myopathy）。上述结果为解析Smyd1缺失如何通过改变转录程序（transcriptional programs）进而诱发中央核肌病提供了重要见解。每组各包含6只动物（对照组与Smyd1条件性敲除组）