102-Plex Approach for Accurate and Multiplexed Proteome Quantification

Hyperplexing approaches have been aimed to meet the demand
for
large-scale proteomic analyses. Currently, the analysis capacity has
expanded to up to 54 samples within a single experiment by utilizing
different isotopic and isobaric reagent combinations. In this report,
we propose a super multiplexed approach to enable the analysis of
up to 102 samples in a single experiment, by the combination of our
recently developed TAG-TMTpro and TAG-IBT16 labeling. We systematically
investigated the identification and quantification performance of
the 102-plex approach using the mixtures of E. coli and HeLa peptides. Our results revealed that all labeling series
demonstrated accurate and reliable quantification performance. The
combination of TAG-IBT16 and TAG-TMTpro approaches expands the multiplexing
capacity to 102 plexes, providing a more multiplexed quantification
method for even larger-scale proteomic analysis. Data are available
via ProteomeXchange with the identifier PXD042398.