Transcriptome profiling of porcine testis reveals genes related to sperm hyperactive motility

The aim of this study was to use RNA sequencing to investigate gene expression differences in testis tissue from Landrace and Duroc boars with high and low levels of sperm hyperactive motility. Twelve gene ontology terms were enriched in the day 0 contrast in Landrace and the most significant term was “extracellular exosome”. For the 96 hours storage contrast in Landrace, seven gene ontology terms were enriched and the most significant one was “extracellular space”. Boars with divergent phenotypes were selected based on their sperm hyperactivity values at the day of ejaculation (day 0) and after 96 hours liquid storage at 18ºC (difference in value between day 0 and 4).