Summary of the localization, vesicle-mediated transport steps, vacuole fusion, and Syb1 localization in membrane trafficking defective mutants.

*Group I indicates that similar to wild-type cells GFP-Syb1 was visibly seen on the plasma membrane with more prominence at the growing ends of the cells.Group II indicates that GFP-Syb1 accumulated as large dot-like structures in the cytoplasm.Group III indicates that GFP-Syb1 localized almost evenly all around the cell surface, and the punctate structures in the cytoplasm were barely observed.Group IV indicates that GFP-Syb1 failed to localize to the membrane and the fluorescence was weak and hazy.