Ets1 suppresses atopic dermatitis by suppressing pathogenic T cell responses

To identify the molecular mechanisms responsible for enhanced atopic dermatitis (AD) pathogenesis upon Ets1 deficiency in CD4+ T cells, we compared transcriptome profile between CD4+ T cells from littermate control (LMC) and Ets1ΔdLck mice at the peak of AD progression by performing RNA-seq. Skin-draining lymph nodes near sites of inflammation under AD were excised from LMC and Ets1ΔdLck mice and prepared into single cell suspensions. CD4+ T cells were purified by CD4+ negative selection method.