Effect of Docosahexaenoic acid on bovine granulosa cells

Lipids in follicular fluid is important environment for oocyte growth. Poly unsaturated fatty acid (PUFA) consist of about half of the fatty acid in human follicular fluid. To see if poly unsaturated fatty acid affect follicle development, granulosa cells obtained from small antral follicles (3-5mm in diameter) of bovine ovaries were cultured with or without 0.1 micromolar of docosahexaenoic acid (DHA) for 2 days, and then RNA was extracted from the cells. This data is RNAseq of the granulosa cells. Exosome RNA was extracted from the granulosa cells using a RNAqueous kit (Life Technologies, Carlsbad, CA, USA) and three batch of RNA were created using differential ovary series. RNA quality was confirmed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), and cDNA libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA). Library quality and quantity were determined using the Agilent 2100 Bioanalyzer and the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA), respectively. A multiplexed library was sequenced as 75-bp single reads on a NextSeq500 platform (Illumina, San Diego, CA, USA). Sequence data were filtered to discard the adapter sequence, ambiguous nucleotides and low-quality sequences. The remaining sequence data were aligned to the Bos Taurus genome sequence (ARS-UCD1.2/bosTau9) to count sequence reads.