Draft genome and annotation of Raphidonema monicae

Microalgae synthesize diverse lipids that perform a wide variety of structural, metabolic, and signalling roles in the cell. However, we know relatively little about lipid metabolism across different protist groups, and especially those adapted to extreme environments. As part of a project to investigate the lipid metabolism of Raphidonema monicae strain SAG 2030, a cold-adapted microalga isolated from Antarctica, a draft genome was assembled and annotated.

The genome was assembled from a single DNA library sequenced with Illumina 150 bp paired-end reads and assembled by NovoGene (Hong Kong) with SOAPdenovo2. Novogene also perfomed curation and structural annotation of CDS and peptide sequences. The data were subsequently functionally annotated in our lab using BlastP, InterProScan and emapper.py, and the results were curated with OmicsBox software.

The assembly sequence and annotation are provided as data files supporting the lipidomic investigation of the effects of abiotic stress and include the following:

File 1. ‘KAD1.seq’ is the assembled genome sequence contigs that have been curated by NovoGene, .fasta format.

File 2. ‘KAD1.gff’ is the annotation file .gff format for the gene CDS, .gff format.

File 3. ‘KAD1.cds’ contains the CDS nucleotide sequences, .fasta format.

File 4. ‘KAD1.pep’ The corresponding peptide sequences, .fasta format.

File 5. ‘kad.annotation.table.xls’ is the combined functional annotation of the peptide sequences using BlastP, InterProScan and Emapper.py in an excel-readable table format.