BMDMs were generated via differentiation of bone marrow cells in culture media containing m-CSF for 7 day. Upon differentiation, BMDMs were left untreated or stimulated with LPS, IFNa, PGE2, LPS+PGE2,
Purity of CD8+CD28? and CD8+CD28? T cells. CD28? and CD28+ CD8+ cells for adoptive transfer studies were sorted by flow cytometry as described in the Materials and Methods section and evaluated for th
