Influence of oleic acid on rumen fermentation and fatty acid formation in vitro.doc

A series of batch cultures were conducted to
investigate the effects of oleic acid (OA) on in vitro ruminal dry matter degradability (IVDMD), gas production,
methane (CH4) and hydrogen (H2) production, and
proportion of fatty acids. Rumen fluid was collected from fistulated goats,
diluted with incubation buffer, and then incubated with 500 mg Leymus chinensis meal supplemented with
different amounts of OA (0, 20, 40, and 60 mg for the CON, OA20, OA40 and OA60
groups, respectively). Incubation was carried out anaerobically at 39℃ for 48 h, and the
samples were taken at 12, 24 and 48 h and subjected to laboratory analysis.