RNA-seq transcriptomics and DIA proteomics of OX-ZmPDI transgenic plants in Zoysia matrella

Zoysia matrella is one of three most economically important Zoysia species due to its strong salt tolerance and wide application. However, the molecular mechanisms regulating salt tolerance of Z. matrella remains unknown. A protein disulfide isomerase ZmPDI of Z. matrella is obtained by salt stress screening with yeast cells, and its expression is significantly up-regulated after salt stress. Based on the obtained ZmPDI overexpression transgenic Z. matrella plants, we carried out salt tolerance identification and found that ZmPDI can significantly enhance the salt tolerance of Z. matrella. Root samples of OX-ZmPDI transgenic and wild-type plants were collected at 0 h and 24 h after salt treatments for RNA-seq and DIA proteome sequencing. This research presents the molecular regulation mechanism of ZmPDI gene in Z. matrella for the resistance of salt stress and facilitates the use of molecular breeding to improve salt tolerance of grasses.