Effects of short-chain fatty acids in inhibiting HDAC and activating p38 MAPK are critical for promoting B10 cell generation and function

Purpose:RNA-seq was uesd to identify how sodium butyrate regulated murine Bregs differentiation. Methods:CD19+ B cells isolated from C57BL/6 mice spleen were were polarized to Bregs differentiation with LPS (10μg/ml) in the absence or presence of sodium butyrate (0.5mM) for 48 hours, and treated with PIM at the last 5 hours following RNA preparation. Results:Using an optimized data analysis workflow, we identified 1462 differentially expressed genes (DEG), of which 720 genes were up-regulated and 742 genes down-regulated, respectively (fold change >2 and adjust p-value < 0.05). RT-qPCR wasused to confirm the reliability of RNA-seq data. KEGG and GSEA analysis indicated that MAPK signaling pathway might involve the function of butyrate on Bregs,which had been proved by western blotting. Using an optimized data analysis workflow, we identified 1462 differentially expressed genes (DEG), of which 720 genes were up-regulated and 742 genes down-regulated, respectively (fold change >2 and adjust p-value < 0.05). RT-qPCR wasused to confirm the reliability of RNA-seq data. KEGG and GSEA analysis indicated that MAPK signaling pathway might involve the function of butyrate on Bregs,which had been proved by western blotting. Using an optimized data analysis workflow, we identified 1462 differentially expressed genes (DEG), of which 720 genes were up-regulated and 742 genes down-regulated, respectively (fold change >2 and adjust p-value < 0.05). RT-qPCR wasused to confirm the reliability of RNA-seq data. KEGG and GSEA analysis indicated that MAPK signaling pathway might involve the function of butyrate on Bregs,which had been proved by western blotting. Bregs were were generated with LPS (10μg/ml) in the absence or presence of sodium butyrate (0.5mM) for 48 hours, and treated with PIM at the last 5 hours.