RNA-Seq analysis, transcriptome assembly and gene expression profile analysis for vascular smooth muscle cells with upregulation of RBM10

To understand the role of RBM10 in the apoptosis of vascular smooth muscle cells (VSMC), we quantified the whole genome transcriptomes of VSMC that were treated by Rbm10 adenovirus (n=3) or control (n=3) for 24 h using RNA-seq. In total, we obtained over 60 million clean reads from each library after trimming adaptor sequences, low quality reads and multiple mapped reads. The clean reads were mapped to 58337 genes annotated in the human reference genome. Human aortic smooth muscle cells were incubated with Rbm10 adenovirus and control overnight and cultured for 24 hours. Total RNA were extracted and generated by deep sequencing, in triplicate, using Hiseq X Ten sequencer.