RNA-seq for traumatic brain injury mice

C57BL/6 male mice (6-8 weeks) were obtained by the Animal Experimental Center of Chongqing Medical University. A controlled cortical impact (CCI) was performed on mice to establish the TBI model. Mice were anesthetized initially with 5% isoflurane and maintained with 1.5% isoflurane in oxygen-enriched air (20% oxygen/80% air) with spontaneous ventilation. CCI injury was induced with an electronically controlled pneumatic impact device (PSI, USA). Briefly, a 5 mm diameter craniotomy was performed on the central aspect of the left parietal bone. The compressed air is driven flat-tip impactor was positioned to the exposed dura's surface and set to impact the cortical surface at a velocity of 5.0 m/s, depth of 2.5 mm, and impact duration 100 ms. Sham animals received the same isoflurane anesthesia, skin incision, and craniotomy, but no impact. All animals resuscitated on an electric blanket maintained at 38 Celsiusafter surgery.3 TBI and 3 Sham mice were included for the RNA-seq.