Whole genome sequencing of multi-drug resistant Pseudomonas aeruginosa collected from hospital environment and clinical isolates.

Antibiotic resistance (ABR) has raised concerns about treatment burden, patient care costs, and morbidity that are of global significance. Studies conducted globally and regionally during the past 20 years have shown a sharp increase in the emergence of carbapenemases in Enterobacteriaceae and other harmful bacteria, including Pseudomonas aeruginosa. P. aeruginosa is a gram-negative bacteria that has been described as one of the most prevalent nosocomial infectious agents. Animals and the environment both contain it. Furthermore, the bacterium's ability to form biofilm, secondary metabolites, and enzymes that enhance its resistance against multiple antibiotic classes, including third-generation beta-lactam antibiotics, is being studied. In order to ascertain the incidence of antibiotic-resistant genes among P. aeruginosa isolates from hospitals and clinical settings, this study will investigate the molecular and epidemiological features of the organism.To perform a comparative study of the ABR gene prevalence patterns and antibiogram profiles of the isolates obtained from patient specimens and hospital ambient samples.Using whole genome sequencing (WGS), determine the common target ABR gene-associated alterations across environmental and clinically prevalent P. aeruginosa.