Characterization of glyceollins as novel aryl hydrocarbon receptor ligands and their role in cell migration

This study is to investigate the effects of glycellin I (GI) and glyceollin II (GII), a aryl hydorcarbon (AhR) ligand in genome. Cells were treated during 24h with glyceollins alone or in combination with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Total RNA was prepared and controlled the quality. Total RNA was prepared and controlled the quality. Then, a 3'Digital Gene Expression (DGE) library was constructed using a modified SCRB-Seq protocol [55] with barcoded poly-dT RT primers and a hybrid Nextera/TruSeq sequencing strategy. Data quality control and data preprocessing were performed by the GenoBiRD Platform in Nantes, France. The results showed that there are numerous gene regulated by TCDD are also modified by GI or GII. GI et GII can have antagonistic or synergistic effects together with TCDD. This is the first study elucidating the effect of GI and GII in three cell types. Method: Cells were treated during 24h with glyceollins alone or in combination with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Total RNA was prepared and controlled the quality. Total RNA was prepared and controlled the quality. Then, a high-Throughput mRNASeq library for 3'Digital Gene Expression (DGE) was constructed using a modified SCRB-Seq protocol [55] with barcoded poly-dT RT primers and a hybrid Nextera/TruSeq sequencing strategy. Data quality control and primary analysis were performed by the GenoBiRD Platform in Nantes, France. Overall design: Examination the effect of glycellins in three cell types: MDA-MB-231, HepG2 and MCF-7