Editing of core genes for strigolactone biosynthesis in tomato

The aim of this work was to generate CRISPR/Cas9 tomato knock-out lines for the SlD27 gene, as well as three other key genes involved in SL biosynthesis (SlCCD7, SlCCD8, SlMAX1), all within the same genetic background. The edited lines exhibited a marked reduction in SL content in root exudates, along with impaired broomrape seed germination. A comprehensive analysis of morphological, reproductive, and fruit-related traits revealed gene-specific effects on plant phenotype, including vegetative traits, fruit set, fruit development, and volatilome. The Sld27 lines, produced for the first time in this study, displayed a phenotype similar to the control non-edited plants, suggesting that the D27 gene holds promise as a breeding target for enhancing resistance to parasitic weeds in tomato. The dataset includes Sanger sequencing of PCR products generated by amplifying target sites and predicted off-target sites of (2) Sld27, (3) Slccd7, (3) Slccd8 and (2) Slmax1 biallelic homozygous lines. Sanger sequences of non-edited control plants (wild type, Wt) are also included. All the files are in ZTR format (Bonfield and Staden 2002)