Spatial Multimodal analysis (SMA) - Mass Spectrometry Imaging (MSI)

The files above are MALDI-MSI (Matrix Assisted Laser Desorption Ionization - Mass Spectrometry Imaging) experiments. The dataset contains both imzML, which is the common MSI open file type, and csv, containing x, y positions in tissue as well as m/z and intensity, which is a more user friendly format. A major difference between the two types is that the imzML contains raw data, whereas the csv data is peak-picked, explaining the discrepancy in file sizes. The data contained in this dataset should be enough to recreate the MSI parts of the manuscript.

The purpose of the experiments was to demonstrate that MALDI-MSI and ST (Spatial Transcriptomics) could be done on the same tissue. MALDI-MSI was done first, and ST second, so there is no expected difference in the tissue compared to a regular MALDI-MSI run, however, the experiments where ST was run subsequent to MALDI-MSI required non-conductive glass which is not the standard in the MSI- field. To compare, experiments done on the commonly used conductive (ITO) glass slides are also contained in this dataset

For any questions about the dataset or to request access to any files not uploaded here, please contact [e-mail]

上述文件为基质辅助激光解吸电离-质谱成像（MALDI-MSI）实验数据。该数据集包含两种格式：imzML，其为常见的MSI开放文件格式，以及csv，其中包含组织中的x、y位置以及m/z和强度，后者是一种更为用户友好的格式。两种类型之间的主要区别在于，imzML包含原始数据，而csv数据则是经过峰值选择的，这解释了文件大小的差异。该数据集中的数据足以重新创建手稿中的MSI部分。

实验的目的是证明在相同的组织中可以同时进行MALDI-MSI和空间转录组学（ST）实验。首先进行MALDI-MSI实验，然后进行ST实验，因此与常规的MALDI-MSI运行相比，组织中不应存在预期差异。然而，在MALDI-MSI之后进行ST实验的实验需要非导电玻璃，这在MSI领域并非标准。为了进行比较，本数据集还包含了在常用导电（ITO）玻璃载片上进行的实验。

如对数据集有任何疑问或需请求访问此处未上传的任何文件，请联系[e-mail]。