Range-wide climate risk and adaptive potential in an iconic cold-water species (Part 2/2)

Predicting extinction risk from climate change requires understanding adaptive variation and local adaptation across species’ ranges. We combine experimental and -omics approaches with climate change modeling to identify molecular mechanisms of local adaptation to heat stress in brook trout, a coldwater species experiencing extirpations due to warming temperatures. We identify genomic variation corresponding with thermal conditions across the native range, suggesting local adaptation, and experimentally identify variants linked with gene expression responses to thermal stress. Using climate projections, we find that southern brook trout populations are the most vulnerable to extirpation from climate warming and mid-range populations are the most promising candidates for receiving assisted gene flow to improve climate resilience. Together, this work highlights the importance of genomic information in managing populations threatened by climate change. This is Part 2 of the full dataset, ..., , # Range-wide climate risk and adaptive potential in an iconic cold-water species (Part 2/2)

Dataset DOI: [10.5061/dryad.nk98sf84m](10.5061/dryad.nk98sf84m)

This is one of two datasets related to the same study:

* Part 1 contains all of the RADseq data, [https://doi.org/10.5061/dryad.sj3tx96fs](https://doi.org/10.5061/dryad.sj3tx96fs)
* Part 2 (this data) contains all of the RNAseq data, [https://doi.org/](https://doi.org/10.5061/dryad.4qrfj6qgn)[10.5061/dryad.nk98sf84m](10.5061/dryad.nk98sf84m)

## Description of the data and file structure

The code and RNAseq data associated with Meek et al. Nature Communications 2025 manuscript.

Zipped files:

* PLATE_1.zip 79.26 GB - contains the demultiplexed RNAseq sequences for each individual in Plate 1. The sample ID of the individual is in the file name (e.g. \"44B*\").
* PLATE_2.zip 63.53 GB - *contains the demultiplexed RNAseq sequences for each individual in Plate 2. The sample ID of the individual is in the file name (e.g. \"44B*\").
* PLA...,