Alfalfa genotyping-by-sequencing (GBS) data

Alfalfa (Medicago sativa L.) quantitative trait loci
(QTL) mapping population (184 F1) derived from cultivars 3010
(cold-tolerant) as female parent and CW 100 (cold-sensitive) as male
parent were genotyped using genotyping-by-sequencing (GBS). Polymorphic
SNPs unique to either 3010 (AB x AA) or CW 1010 (AA x AB) were identified
as single dose allele (SDA) markers and used to generate the genetic
linkage maps. Two sets of linkage maps, a set for each parent, were used
to map the traits and the QTL were identified. With the genotyping and
phenotyping informations we were able to map various alfalfa traits such
as fall dormancy, winter-hardiness, freezing tolerance, flowering time,
yield and leaf-rust resistance. The raw sequence data were deposited at
NCBI SRA with the accession number SRP150116. This study identified
several genomic regions and associated markers that can be further
utilized in marker-assisted breeding to improve the alfalfa.