Supplementary material for manuscript submission at Ecology and Evolution

Supplementary data for the manuscript submission at Ecology and Evolution entitled: "<i>Gaps and data ambiguities in DNA reference libraries: A limiting factor for molecular-based biodiversity assessments using Arabian Peninsula annelids as a case study</i>"<b>Checklists:</b>Arabian Checklist (CL-MTAP)Mediterranean Checklist (CL-MTMS)Polychaeta Checklist from the Arabian Peninsula (Wehe and Fiege 2002; CL-MTAWF)<b>BOLD Annelida FASTA COI dataset:</b>DS-MTWA<b>ARMS Metabarcoding ASV obtained FASTA dataset:</b>DS-ARMS<b>Supplementary Tables and Figures:</b><b>Table S1</b><b>:</b> Obtained ASVs and respective number of reads per ARMS and respective taxonomic identification<b>Table S2</b><b>:</b> ARMS metadata with information regarding number of ARMS deployed by region, year and respective coordinates.<b>Fig. S1</b><b>:</b> The number and percentages of species (A), genera (B) and families (C) from the Annelida Mediterranean checklist (CL-MTMS) with available DNA barcodes in the BOLD database. (D) Number of available sequences for the most represented families with DNA barcodes in CL-MTMS. (E) Number of barcoded species and species yet to be barcoded for the top10 most represented families in CL-MTMS.<b>Fig. S2</b><b>:</b> Neighbour-Joining phylogenetic Tree using Kimura 2-parameters model for DS-ARMS. All 5375 ASVs are individually shown with the respective automatic taxonomic assignment. Discordant clades (based on ASAP methodology) highlighted in red.Public DOIs for specific BOLD related datasets will be available upon paper acceptance.