Transcriptomics of standardized 3D bioconstruct constituted by fibroblasts and keratinocytes in a collagen matrix.

The therapeutic usage of physical stimuli is framed in a highly heterogenous research area with variablelevels of immaturity and translatability into medical application. In particular electrostimulation is deeplystudied for its application on the autonomous nervous system, but little is known about the anti-inflammatory effects of such stimuli beyond the inflammatory reflex. Further, reproducibility and meta-analyses on existing results are extremely challenging owing to the limited rationale on dosage andexperimental standardization.In this work we propose a series of controlled experiments on the effects of a number of electrical stimuli(in direct and alternate current) delivered on a standardized 3D bioconstruct constituted by fibroblasts andkeratinocytes in a collagen matrix.Transcriptomics at selected time points allow to obtain a clearer picture of thebiological mechanisms at stake, highlighting the differential anti-inflammatory potential of suchapproaches in selected conditions.We hope that our results will trigger an interest and a facilitation in the study of the anti-inflammatoryeffects of physical stimuli, highlighting not only the potential but also the limitations of such approaches,offering, ultimately, solid evidence for future translation into the clinic.