beta 2 Adrenergic receptor expression analysis by flow cytometry on CD4+ T cells isolated from mPGES-1 KO or WT mouse splenocytes

Flow cytometric data from five experiments analyzing beta-2-adrenergic receptor expression on CD4+ T cells (MACS positive selection from spleen) from mPGES-1 ko (-/-) or WT (+/+) mice subjected to vagus nerve stimulation (VNS) and intraperitoneal LPS injection 30 min prior to sample collection.Panel (more detailed info can be found in the "FCS file identification list" document):FITC-ViabilityPE-b2ARPCPCy5.5-CD69PECy7-CD62LAPC-CD44APCCy7-CD3BV421-CD25BV510-CD4The protocol for staining, cell extraction VNS etc. is described in the publication "Microsomal prostaglandin E synthase-1 gene deletion impairs neuro-immune circuitry of the cholinergic anti-inflammatory pathway in endotoxaemic mpuse spleen" published in PLOS ONE 2017 by the authors: Priya Revathikumar, Johanna Estelius, Utsa Karmakar, Erwan LeMaître, Marina Korotkova, Per-Johan Jakobsson and Jon Lampa.