Effects AGN193109 on endoderm marker gene expression.

Inhibitor was applied to the culture medium; Stage indicates the stage of treatment; ND, not done;
Phenotypes: Cyp26A1, expression is inhibited in discrete areas (see text); HoxB4, posterior shift of expression domain and decreased expression level; Hex, comprise embryos collected at HH 5–6 and HH 14; at HH 5–6, Hex is reduced and at HH 14, Hex is ectopically expressed between thyroid & liver; Nkx2.1, HoxA2 and Prox1 no change in expression pattern. γ−fibrinogen, slight increase in expression. Pdx1, complete repression; CdxA, two phenotypes1 partial or complete repression or2 anterior expression border is posteriorly shifted.