Reconstructing aspects of human embryogenesis with pluripotent stem cells

We have established an in vitro model that reconstructs aspects of human embryogensis using human expanded potential pluripotent stem cells (hEPSCs). We performed single-cell RNA sequencing (scRNA-seq) on hEPSCs grown in 2D (prior to aggregation), hEP-structures at day 5, hEP-structures grown in IVC for 24h, and natural human blastocysts at day 5/6. Overall design: Single cell transcriptome analysis of hEPSCs grown in 2D (2 replicates), hEP-structures at Day 5 (3 replicates), hEP-structures at Day 6 (3 replicates), and D5/D6 natural human embryos (6 embryos).