Sterol peaks identification and composition (%) found by GC-MS in polyene (AmB and Nys) resistant lines of Leishmania spp.

A) Identification of sterol peaks by GC-MS. Standards used were: cholesterol, TMS; desmosterol, TMS; 5α-cholest-7-en-3β-ol, TMS; ergosterol, TMS; stigmasterol, TMS; β-sitosterol, TMS; lanosterol, TMS; FF-MAS (4,4-dimethyl-5α-cholesta-8,14,24-trien-3ß-ol) and zymosterol. FF-MAS: Follicular fluid meiosis-activating sterol and an intermediate in the cholesterol biosynthetic pathway present in all cells. Peaks that did not match any standard were determined by comparing with the NIST spectral libraries with the ion trap mass spectrometer. 5-dehydroepisterol based on literature [100,126,132,174]. RT: retention time. TMS: trimethylsilyl (for derivatization). B) Sterol composition (%) by GC-MS in polyene (AmB and Nys) resistant lines of Leishmania spp. promastigotes and amastigotes. Content of each sterol is the percentage of the total of the raw peak area detected per line ± Standard deviation of three independent biological replicates. Sterol content is shown as the total (%) of the raw peak area detected per sample (± SD) from all biological replicates (n = 3). (XLSX)