Data for "Not dear neighbours in the ant Lasius niger"

This README accompanies the files "Rmarkdown_Cordonnier.pdf", “data_final.txt”, and “Genotypes.xlsx” Rmarkdown_Cordonnier.pdf ********************************** CONTENTS *********************************** Rmarkdown for ‘Not dear neighbours: Antennation and jerking, but not aggression, correlate with genetic distance and physical distance in the ant Lasius niger’ Data_final.txt ********************************** CONTENTS *********************************** Dataset associated with the Rmarkdown The data are in table form with TABs as variables field delimiters so they can be readily imported in any statistical package or spreadsheet program. Please, contact me if you need the file formatted otherwise. This file includes a description of the variables. ******************************************************************************* Variable names and descriptions date date of the experiment time time of the experiment temp_outdoor outdoor temperature during the experiment temp_indoor indoor temperature during the experiment pair_ID ID of the pair of individuals tested in the experiment target_col ID of the target colony tested in the experiment comp_col ID of the competitor colony tested in the experiment distance geographical distance between the colonies involved in the experiment group allospecific (interactants from different species), nestmate (interactants from the same species, same colony), non-nestmate (interactants from same species, different colonies) neighbour neighbour interactants (adjacent slabs) versus non-neighbour interactants neutral_body_contact number of neutral body contact between the ants during the interaction antennation number of occurrences of antennation during the interaction jerking number of occurrences of jerking during the interaction mandible_gaping number of occurrences of mandible gaping during the interaction brief_biting number of occurrences of brief biting during the interaction gaster_flexing number of occurrences of gaster flexing during the interaction fighting_prolonged_biting number of occurrences of fighting or prolonged biting over the interaction RQG_Col relatedness between pairs of colonies (estimator of Queller & Goodnight) Genotypes.xlsx ********************************** CONTENTS *********************************** Genotypes of the workers IDcolony ID of the source colony of the genotyped worker Ant1343 to ant2794 list of the 11 microsatellite markers used in the study The 15 µl PCR reaction volume consisted of 7.5 µl GoTaq® G2 Hot Start Colorless Master Mix (M7433, Promega), 4.5 µl ddH2O, 1.0 µl unlabelled reverse primer (10µM), 1.0 µl labelled forward primer (10µM; labelled HEX, FAM and TET; final concentration of 0.67µM for each primer) and 1 µl DNA (2-10 ng). The PCR consisted of initial denaturation at 94°C (4 min), 33 cycles at 94°C (denaturation, 30 s), 55°C (annealing, 30 s) and 72°C (elongation, 30 s), and a final step at 72°C (1 min). The PCR products were analysed in an ABI PRISM 310 Genetic Analyser (PE Biosystems) after DNA denaturation at 90°C (1 min). Allele sizes were determined using genescan 3.1 software (PE Biosystems). ********************************* CONTACT ********************************** Please contact me at: Marion Cordonnier e-mail: [e-mail] *******************************************************************************