Perturbation of healthy iPSC-CM with hsa-miR-134, hsa--433, and hsa-487b mimic and inhibitor

In this study, healthy iPSC-CM were transfected with hsa-miR-134, hsa--433, and hsa-487b mimic and inhibitor to study their effects on human cardiac proliferation after it was found that these miRNAs were enriched in maternal blood plasma within SVHD pregnancies. Mock and scramble were used as controls. Overall, it was found that miR134 and miR487b influenced human iPSC-CM proliferation. Overall design: iPSCs were differentiated in to iPSC-CM via small-chemical modulation of Wnt. D15 iPSC-CM were passaged onto a 24-well plate and then transfected with miRNA mimic, mock, scramble, or inhibitor in experimental triplicates. Four wells were pooled for each group and lysed with Qiazol. Total RNA was extracted four days after transfection, with the results of each being the subject of this RNA-seq data.