Mouse vocal fold permeability in vivo: Effects of novel low-tech injury and instillation methods

Objectives: Vocal fold (VF) injury and instillation are emerging techniques to study laryngeal pathologies in mouse models. Most approaches require high-tech visualization and lengthy anesthesia. Intraperitoneal (IP) naphthalene (NAPH) and topical polidocanol (PDOC) are two chemical methods of injuring murine lower airways. Oropharyngeal aspiration (OA) is used for murine lung instillation. We assessed whether these simple low-tech methods could injure and instill mouse VFs, and whether injury increased in vivo VF epithelial permeability.
Methods: Mouse VFs were injured using IP NAPH at 200, 250, 300, or 350 mg/kg, or OA of PDOC at 0.5% or 2% w/v. Twenty-four hours later, mice received Evans blue (EB) dye OA instillation, then were sacrificed after 30 minutes. Coronal larynx sections were assessed for VF injury. Permeability was measured by imaging EB autofluorescence and quantifying percent positive area and signal intensity. VFs were immunostained for basal cells (p63), tight junction..., , # Data from: Mouse vocal fold permeability in vivo: Effects of novel low-tech injury and instillation methods

Dataset DOI: [10.5061/dryad.9w0vt4bsc](10.5061/dryad.9w0vt4bsc)

## Description of the data and file structure

The dataset contains microscopy images and data used to generate the graphs and statistical analyses in figures in the manuscript. Images (.TIFF) and data files (.xls) can be viewed and/or analyzed with a number of free or proprietary software programs.

Image naming structure: sample#_slide#_tissue_magnification_filter/stain.

### Files and variables

#### File: Figure_1.zip

**Description:** 

1. HE folder: Images used to create Figure 1A-C and analyzed to generate data for Figure 1D. Brightfield images of hematoxylin and eosin (H&E)-stained vocal folds, arranged in folders by injury.
2. Figure 1 data.xls: Data underlying Figure 1D. Columns: injury group (categorical), VF sloughing (number of animals per group), no VF sloughing (number of animals per group), % with ...,