Supplemental Material for Sorgini et al., 2021

Supplemental Tables S1-S5.Table S1: Recombinant Inbred Lines used for single family QTL mapping of leaf δ13C, SLA, and ionomics.Table S2: Recombinant Inbred Lines used for leaf δ13C joint linkage mapping.Table S3: Lines used for leaf δ13C GWAS.Table S4: Significant QTL identified in ionomics analyses.Table S5: Weather data for field sites.<br><b>Supplemental Figure 1 NAM RIL Transgressive Segregation. </b>NAM RIL families CML103 (<b>A</b>), CML333 (<b>B</b>), NC358 (<b>C</b>), and Tx303 (<b>D</b>) were sorted by δ¹³C and plotted. Parental lines are shown in red. <b>Supplemental Figure 2 Element and δ¹³C Full Correlation Matrix.</b> A full correlation matrix of element and δ¹³C mean values is show. The diagonal displays histograms of each dataset. Pearson’s r is shown in the upper panel. Scatter plots and best fit line are shown in the lower panel. <b>Supplemental Figure 3 Element QTL Mapping by Chromosome. </b>Significant element QTL are shown by maize chromosomes 1 through 10 on the x-axis (in cM). Each NAM RIL family is represented by a symbol; CML103 (○), CML333 (x), NC358 (□), and Tx303 (Δ). Each element is designated by color. Significance thresholds (dashed horizontal line) were determined using 200 permutations, alpha=0.05 for each QTL independently. <b>Supplemental Figure 4 Genome Wide Association Study for δ¹³C. </b>Manhattan plot showing significance of SNPs derived from a mixed linear model using the Bayesian information criterion to select the optimal number of principal components. The significance threshold represents the Bonferroni correction of familywise error rate.<br>