Preparation of soil bound HY TME, DY TME^a, and elk CWD^a PrP^Sc.

a SCL bound DY TME and elk CWD PrPSc were prepared with the same soil to brain ratio and in the same condition as SCL bound HY TME. HY TME was also used for animal bioassay b BH, 10% brain homogenate. c SiO2-HA, SiO2-humic acid. d The amount of samples used for PMCA was expressed as weight of soil—equivalent volume of soil solution. e Due to the performance challenge, PMCA substrate was added to the soil pellets of weight as indicated in the table. Preparation of soil bound HY TME, DY TMEa, and elk CWDa PrPSc.