RNA squencing of human NK cells activated with IL-15, a combination of IL-12, IL-15, and IL-18, or with a trimeric fusion protein 18/12/TxM

Gene expression analysis of human NK cells at baseline, Day 1, and Day 6 after activation with IL-15, IL-12/15/18, or the 18/12/TxM molecule NK cells were isolated from 3 distinct human donors (CD56+ CD3-), incubated with low-dose IL-15 (1 ng/mL), IL-12, IL-15, and IL-18 (IL-12 - 10ng/mL, IL-15 - 50 ng/mL, IL-18 50ng/mL), or 18/12/TxM (38.8nM) for 16 hours, washed, and cultured in 1ng/mL IL-15 for 6 days before isolation for sequencing analysis