Nutrition Induced Expression Changes In Eight Clock Genes In Porcine Tissues

The goal of the current work was to investigate if such changes also take place in tissues containing peripheral (duodenum, dorsal fat, muscle, and liver) and central (hypothalamus) clocks. As animal material, we used 12 sows that fasted 12 h before slaughtering (T0) and 12 sows that were fed ad libitum 7 h. prior slaughtering (T2). Tissue samples were collected immediately after slaughter and total RNA was subsequently extracted. The expression of the ARNTL, BHLHE40, CRY2, NPAS2, NR1D1, PER1, PER2 and SIK1 genes was measured by quantitative reverse transcription PCR. <br>