Effect of Gsdma1and Gsdma3 epithelial knockout on gene expression of mouse dorsal skin keratinocytes after olive oil/acetonr treatment to induce epidermal barrier disruption

To investigate the biological fundtion regualted by Gsdma1/a3 during epidermal barrier formation, we perform acetone/olive oil vs. water treatment on the dorsal skin of control and Gsdma1/Gsdma3 epithelial knowckout (GsdA1A3eKO) mice. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 water-treated and 3 aceton/olive oil treated for each genotype (wild type vs. GsdmA1A3eKO samples). Overall design: Comparative gene expression profiling analysis on RNA-seq data of mouse back skin keratinocytes from control and Gsdma1/a3 epitheial knockout mice after topical olive oil/acetone (AOO) or water on dorsal skin