Study the role and function of HP1 in embryonic stem cells

In this article, we first obtained HP1 triple-knockout embryonic stem cells using Crispr-Cas9 and Cre-loxP systems. At the same time, we also obtained single-knock and double-knock cell lines respectively. On this basis, we studied The function of HP1 in embryonic stem cells was found to have a very important role in the regulation of differentiation and reproduction-related genes.In our follow-up study, we found that the deletion of HP1 leads to the differentiation tendency of embryonic stem cells into endoderm. Considering that HP1 has an important function of H3K9me3 recognition and binding, we examined the effect of HP1 deletion on histone modifications, we found that H3K9me2 and H3K9me3 modifications were significantly reduced, and then through rescue experiments, we thought that HP1 may be through OCT4 and NANOG and then further functional. Comparative gene expression profiling of RNA-seq data from HP1 single-knock-down and triple-knock cells versus WT embryonic stem cells. Comparative gene expression profiling of RNA-seq data from SETDB1 knockout cells and WT embryonic stem cells.