Endoderm differentiation of human embryonic stem cells (hESCs) [RNA-Seq]

Purpose: The goal of this study is to identify the whole-genome transcriptome profiling of differentially expressed genes during human embryonic stem cell differentiation. Methods: Triplicate RNA profiles were generated from hESCs and induced endoderm cells, by deep sequencing with Illumina NextSeq 500. Results: We identified 6320 genes differentially expressed between hESCs and induced endoderm cells, with foldchange ? 1.5 and P < 0.05. Conclusion: Our study confirms that endodermal gene were dramatically induced during endoderm differentiation. Overall design: RNA profiles of human embryonic stem cells and endoderm cells induced from ES cells for 3 days were generated by deep sequencing, in triplicate, using Illumina NextSeq 500.