16S rDNA sequencing data for characterizing the endosymbionts of leaf curl plum aphid ( Brachycaudus helichrysi) clones

Asexual lineages often exhibit broad distributions and can thrive in extreme habitats compared to their sexual counterparts. Several hypotheses can be proposed to explain this pattern. Asexual lineages could be versatile genotypes with wide environmental tolerance, enabling their dispersal and persistence across large geographic areas. Alternatively, asexual genotypes could be ecological specialists that thrive in specific environments and outcompete relatives colonizing distantly related areas with similar conditions in the process. Several aphid species feature widespread obligate asexual lineages, commonly known as “superclones”. Yet it is often unknown whether these clones are widespead ecological generalists or  successful specialists. To explore these hypotheses, we examined climatic niche differentiation among six globally distributed obligate asexual lineages of the cosmopolitan aphid pest, Brachycaudus helichrysi. To insure that we were investigating the aphid genotype niche an..., This data results from the sequencing of a 251 bp fragment of the 16S rDNA bacterial gene amplified in a worldwide sample of the leaf curl plum aphid pest (Brachycaudus helichrysi). The table with collection details is also attached to this data submission.
A total of 102 individuals, representative of six aphid clones and the diversity of their geographic distribution were used for characterising bacterial endosymbionts. Using DNA extracts from the individuals utilised for microsatellite genotyping in Piffaretti et al. (2013), we amplified a 251 bp portion of the V4 region of the 16S rRNA gene (Mizrahi-Man et al. 2013) and used targeted sequencing of indexed bacterial fragments on a MiSeq (Illumina) platform (Kozich et al. 2013) following the protocol described in Jousselin et al. (2016). Briefly, each DNA sample was amplified twice (replicates were conducted on distinct 96-well microplates). We also used negative controls (DNA extraction and PCR controls conducted on blank templates) ..., , # 16S rDNA sequencing data for characterizing the endosymbionts of leaf curl plum aphid ( Brachycaudus helichrysi) clones

[https://doi.org/10.5061/dryad.zpc866tgn](https://doi.org/10.5061/dryad.zpc866tgn)

The data set contains raw reads resulting from the illumina sequencing of a fragment of 16S rDNA gene on a world wide Brachycaudus helichrysi sampling.

A total of 102 individuals, representative of six *B. helichrysi* clones and the diversity of their geographic distribution were used for characterising bacterial endosymbionts. Using DNA extracts from the same individuals utilised for microsatellite genotyping in Piffaretti et al. (2013) or Popkin et al. (2017), we amplified a 251 bp portion of the V4 region of the 16S rRNA gene (Mizrahi-Man et al. 2013) and used targeted sequencing of indexed bacterial fragments on a MiSeq (Illumina) platform (Kozich et al. 2013) following the protocol described in Jousselin et al. (2016). Each DNA sample was amplified twice (replicates were condu...