Proteolytic activity of C-SVMP upon FRET substrate.
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https://figshare.com/articles/dataset/_Proteolytic_activity_of_C_SVMP_upon_FRET_substrate_/839019
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资源简介：
The proteolytic activity of C-SVMP (0.5 µg) on the FRET peptide (Abz-FRSSRQ-EDDnp - 5 µM in PBS) was determined in the presence or absence of 15 mM of phenylmethylsulfonyl fluoride (PMSF) or 1,10 phenanthroline (Phen). Reactions were carried out at 37°C using a fluorescence spectrophotometer (λem = 420 nm and λem = 320 nm). All assays were performed in duplicate and the specific proteolytic activity was expressed as micromoles of cleaved substrate per minute per µg of protein (U/µg).
创建时间：
2013-10-31
