Rhesus Macaque Trophoblast Organoids Single-Cell and Single-Nucleus RNA Sequencing

Trophoblasts are fetal-derived cells responsible for nourishing and protecting the developing fetus and are a universal component of all placentas. While primate placentas exhibit many shared morphological features, species-specific differences in gene expression remain largely unexplored, primarily due to the lack of suitable in vitro models. To address this gap, we developed trophoblast organoids from rhesus macaque placentas, and compared them with organoids derived from human placentas. We performed comparative single-cell RNA sequencing and single nucleus RNA sequencing to delineate shared and distinct transcriptional signatures between rhesus macaque. Four samples of rhesus trophoblast organoids were submitted for single-cell RNA sequencing. Single-nucleus RNA Sequencing was performed on three samples of rhesus trophoblast organoids grown under normal conditions and three samples of EVT enriched rhesus trophoblast organoids. All trophoblast organoids were grown according to established trophoblast protocols. Organoids were derived from 3 unique lines.