List of primers and PCR conditions used.
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All primers used in this study are given in 5’-3’ direction. Annealing temperature, extension time and expected band size given. Note: for gfp-rx2 two major bands are expected upon HDR-mediated integration–the wt size of non-gfp-integrated loci as well as the larger single-gfp insertion. Rx2 tagging design is as described in Gutierrez et al., 2018 (20).
创建时间：
2019-03-05
