Transcriptome of Peromyscus leucopus lungs infected with SARS-CoV-2

Peromyscus leucopus is a reservoir for numerous zoonoses and one of the most abundant mammals in North America. Since they live near humans we are interested in the possibility of SARS-CoV-2 infection in this animal model from a zoonoses standpoint. The experimental design for the first trial involves using adult male and female P. leucopus LL stock. Animals were dosed with nasal inhalation of either tissue culture medium (DMEM) alone (controls) or alpha variant, WA1 strain of SARS-CoV-2 virus. We dosed 8 animals with a virus (titer 2x104) and 6 with DMEM alone. Animals were euthanized at either 3 or 6 days post-infection. For a second trial, P. leucopus animals were 1-3 years old and we had 3 groups: control animals with nasal inhalation of media, control animals with nasal inhalation of the heat-treated virus, and infected animals. Each group has 4 animals euthanized on 3- or 6-days post-infection (DPI). Lung tissue was used for RNA isolation and further sequencing to obtain 40 x 106 ..., Lung RNA was isolated using Direct-zol Miniprep kit according to instructions. Lung RNA was sequenced using Illumina technology. The stranded mRNA library was normalized, multiplexed, and sequences on the NovaSeq6000 instrument at the UC Irvine genomic High Throughput Facility. We used paired-end chemistry and 150 cycles to achieve 40 million reads per sample. 
The project and the description of the samples are described under the following NCBI BioProjects: PRJNA1026365 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1026365). Manuscript in preparation; URL pending., , GENERAL INFORMATION

1. Title of Dataset: Transcriptome of Peromyscus leucopus lungs infected with SARS-CoV-2
2. Author Information
A. Principal Investigator Contact Information

Name: Alan G. Barbour

Institution: University of California Irvine

Address: 843 Health Sciences Court, Irvine, CA 92697

Email: [e-mail]

B. Associate or Co-investigator Contact Information

Name: Ana Milovic

Institution: University of California Irvine

Address: 843 Health Sciences Court, Irvine, CA 92697

Email: [e-mail]
3. Date of data collection (single date, range, approximate date): 2020-2022
4. Geographic location of data collection: Irvine, California, USA
5. Information about funding sources that supported the collection of the data: National Institute of Allergy and Infectious Diseases grants AI-136523 and AI-157513

SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: None
2. Links to publications that cite or use the data...