CRAC of yeast RNA polymerase II in WT and isw1Δ cells in standard conditions or upon ER stress.

We obtained RNA polymerase II occupancy profile across the genome of WT and isw1Δ Saccharomyces cerevisiae strains grown under normal conditions or after ER stress induction for 2H with1 µg/mL tunicamycin (Tm). The nascent RNA was crosslinked to RNAPII (Rpb1) and sequenced after purification of Rpb1 under stringent and denaturing conditions. This reveals the position of the polymerase at the nucleotide resolution level.