The Deciphering of Mouse Uterine Microenvironment during Early Pregnancy [spatial transcriptomics]

The mouse uterine microenvironment at pregnancy D7.5 was investigated by Visium spatial transcriptome. Ten distinct uterine microenvironments were identified with diverse biological functions, different endocrine regulations and intensive interactions. The single cell RNA-seq of D7.5 mouse uterus has been intergrated with the spatial visium results and predicted the presence of mutiple types of cells in each microenvironment. The mouse uterus were collected at D7.5, either sectioned for Visium spatial transcriptome, or isolated into single cell for scRNA-Seq.本研究采用Visium空间转录组（Visium spatial transcriptome）技术，对妊娠第7.5天的小鼠子宫微环境展开系统性探究。研究共鉴定出十种具备多样化生物学功能、差异化内分泌调控模式及紧密细胞相互作用的独特子宫微环境。本研究将妊娠第7.5天小鼠子宫的单细胞RNA测序（single cell RNA-seq）数据与Visium空间转录组结果进行整合，进而预测了每种微环境中所含的多种细胞类型。实验小鼠于妊娠第7.5天处死取材，部分样本经切片后用于Visium空间转录组测序，其余样本则被解离为单个细胞以开展scRNA-Seq测序。