Oct4 mainly controls enhancer activity rather than accessibility
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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资源简介：
The transcription factor Oct4 is essential for stem cell pluripotency and efficient cell reprogramming, but its functional roles remain incompletely understood. Here we investigate Oct4 function by rapidly depleting Oct4 from mouse embryonic stem cells and conducting a time-resolved multiomics analysis. Oct4 depletion leads to an immediate loss of Oct4 binding to putative enhancers that are accessible in chromatin. Oct4 loss goes along with a simultaneous decrease in mRNA synthesis from putative target genes that are part of the transcriptional network that maintains pluripotency. Oct4 binding to enhancers does not correlate with chromatin accessibility, whereas Sox2 can retain accessibility after Oct4 depletion even in the absence of eRNA synthesis. These results are consistent with the model that Sox2 mainly acts as a pioneer factor that renders enhancers accessible, whereas Oct4 mainly acts as a transcriptional activator that stimulates transcription of pluripotency enhancers and their target genes.
提供机构：
MPI for biophysical chemistry
创建时间：
2022-02-20
