Expression data from aortic smooth muscle cells (AoSMCs) from mouse

Chronic kidney disease (CKD) can induce AoSMCs premature senescence and phenotypic switching, which ultimately promotes AS progression and plaque vulnerability. However, the underlying mechanisms are still unknown. To identify the underlying mechanisms of AoSMCs senescence and phenotype switching in CKD. We performed integrated microarray analysis of AoSMCs from normal-diet fed ApoE-/- (ND) mice, high fat-fed ApoE-/- (AS) mice and CKD ApoE-/- (accelerated atherosclerosis mouse model, AAS) mice. AoSMCs were sorted from the mice arteries using flow cytometry. Then, RNA was extracted from sorted AoSMCs and hybridized on Affymetrix microarrays. The groups are as described above: ND, AS and AAS group.