Molecular fingerprint and anti-carcinogenic properties of Turkish immortelle (<i>Helichrysum italicum</i>) essential oil

Immortelle (<i>Helichrysum italicum</i>) essential oil is one of the most valuable essential oils with its commercial importance and favorable characteristic properties in various cosmetic products and food products. Current research performed a comprehensive characterization of Immortelle essential oil using powerful and robust analytical techniques. The relative volatile composition of the Immortelle essential oil from Turkey was determined by GC-MS (gas chromatography-mass spectrometry) technique. Thirty-eight volatile compounds were identified. Average total phenolic content and DPPH radical scavenging activities (IC₅₀) were determined as 54.24 mg GAE/mL essential oil and 0.27 mg/ml, respectively. FTIR and Raman spectroscopy fingerprints were determined for chemical characterization. The thermal profile was obtained by DSC (differential scanning calorimetry) analysis. The Immortelle essential oil (12.5 μg/mL) inhibited the growth of Caco 2 adenocarcinoma cells at the ratio of 48% without any deterioration in healthy L929 fibroblast cells according to the cytotoxicity analyses.

蜡菊（Helichrysum italicum）精油是极具商业价值的精油品类之一，其不仅具备重要的商业应用价值，且在各类化妆品及食品中展现出优良的特性。本研究采用高效可靠的分析技术，对蜡菊精油开展了全面的表征分析：通过气相色谱-质谱联用（gas chromatography-mass spectrometry）技术，测定了产自土耳其的蜡菊精油的相对挥发性成分组成，共鉴定出38种挥发性化合物；其平均总酚含量与DPPH自由基清除活性（半抑制浓度IC₅₀）分别为54.24 mg没食子酸当量/mL精油与0.27 mg/mL；通过傅里叶变换红外光谱（Fourier Transform Infrared Spectroscopy，FTIR）与拉曼光谱获取了该精油的化学表征指纹图谱；通过差示扫描量热法（differential scanning calorimetry，DSC）分析得到了该精油的热学特性曲线。细胞毒性分析结果显示，浓度为12.5 μg/mL的蜡菊精油可使Caco-2腺癌细胞的生长抑制率达48%，且对健康L929成纤维细胞无明显毒性损伤。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于土耳其蜡菊精油的分子指纹和抗癌特性研究，通过GC-MS、FTIR、拉曼光谱和DSC等技术全面表征其化学成分、抗氧化活性及热性质。研究发现该精油含有38种挥发性化合物，并在12.5 μg/mL浓度下能选择性抑制Caco 2腺癌细胞生长，对健康细胞无损害，突显其潜在抗癌价值。