Raw data of the article "Bovine Whey Fractions Restore Epithelial Barrier Function Under TNF-α/IFN-γ Challenge in a Caco-2/HT29-MTX Co-Culture Model"

Description of the project Inflammation-induced intestinal barrier dysfunction is a key feature of several gastrointestinal disorders. Bovine whey contains bioactive components with potential barrier-protective and immunomodulatory properties, although its activity may depend on fraction composition. In this study, the effects of whole bovine whey (BWW) and skimmed bovine whey (BSW) were evaluated in a differentiated Caco-2/TC7:HT29-MTX co-culture model exposed to basolateral TNF-α + IFN-γ (50 ng/mL, 24 h). Cytokine treatment significantly reduced transepithelial electrical resistance (TEER), downregulated CLDN1 and CLDN3, increased IL-1β expression, and decreased MUC2 expression, whereas FITC-dextran permeability remained unchanged. Both whey fractions restored TEER to values comparable to control and also increased TEER under basal conditions. BWW attenuated cytokine-induced IL-1β upregulation, whereas BSW increased MUC2 expression under both basal and inflammatory conditions. These results indicate that BWW and BSW improve epithelial barrier function under inflammatory conditions through distinct functional activities. Description of the dataset This dataset contains the experimental data associated with Figures S2 and 1-4 of the mentioned article. Files included -Raw Data Figure-S2.xlsx :Raw data regarding TEER values for figure S2. -Raw Data Figure-1.xlsx : Raw data regarding TEER values for figure 1. -Raw Data Figure-2.xlsx : Raw data regarding FITC-dextran flux values for figure 2. -Raw Data Figure-3.xlsx : Raw data regarding qPCR values for figure 3. -Raw Data Figure-4.xlsx : Raw data regarding qPCR values for figure 4. -Processed Data Figure-S2.xlsx :Processed data regarding TEER values for figure S2. -Processed Data Figure-1.xlsx : Processed data regarding TEER values for figure 1. -Processed Data Figure-2.xlsx : Processed data regarding FITC-dextran flux values for figure 2. -Processed Data Figure-3.xlsx : Processed data regarding qPCR values for figure 3. -Processed Data Figure-4.xlsx : Processed data regarding qPCR values for figure 4. README.txt — this document