Effect of H2O2 treatment on gene expression of islet derived hybrid cell of 1.1B4. Homo sapiens

We analyzed the comprehensive gene expression changes by H2O2 in pancreatic beta-cell derived 1.1B4 cells using Agilent microarray. The genes of 2903 H2O2 treated were up-regulated and 2283 genes were down-regulated compared with control sample. HMOX1, EGR1, IGF, IL8 and NR4A3 were strongly up-regulated Overall design: Gene regulation associated with antioxidant defense system in pancreatic beta cells still needs to be elucidated, we analyzed the genes expression with Agilent micro-array after 4 hours of 100mM H2O2 treatment by one color method, and found 2903 genes were up-regulated and 2283 genes were down regulated. Changes of some genes expressions were confirmed by real time PCR.